Integrins are cell adhesion molecules that can mediate bidirectional transfer of signals across the plasma membrane. The cytoplasmic domains of integrin family members interact with components of the signal transduction apparatus within cells. Integrin receptors contain noncovalently associated α and β subunits that consist of a large extracellular region (the ligand-binding domain), a short transmembrane region, and a cytoplasmic domain of varying length. In mammals, at least 17 α subunits and 8 β subunits have been identified and these proteins can heterodimerize to form at least 22 different receptors. Integrin α5 and integrin β1 form a receptor for fibronectin and fibrinogen. This receptor is important for vascular development. Both integrin subunits of the fibronectin receptor are heavily glycosylated in their extracellular domains. This modification is essential for proper cell attachment to basal membranes. The integrin β3 subunit associates with integrin α2 in platelets where this glycoprotein complex acts as a fibrinogen receptor and mediates platelet aggregation. Integrin α5 has been reported to suppress apoptosis by a Bcl-2 pathway and the C-terminal region is critical for cell motility and cytoskeletal rearrangements. The integrin β2 subunit associates with integrin αL to form a receptor for ICAM family members, while integrin β3 complexes with the integrin αV subunit to form the vitronectin receptor in endothelial cells.
Iwamoto, D. & Calderwood, D. (2015) Cur Opin Cel Bio. 36:41.
Iwamoto, D, Calderwood, D.(2015) Curr Opin Cell Biol. 36:41.
The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.
*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
Product References:MS3001 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot
This kit contains:
|CATALOG#||DESCRIPTION||SIZE||APPLICATIONS||SPECIES REACTIVITY||MW (kDa)|
|Integrin α2 (Extracellular region) Mouse mAb||50 μl||WB, E||Hu, Rt, Ms||150|
|Integrin α5 (Extracellular region) Mouse mAb||50 μl||WB, E, IP||Hu||150|
|Integrin αL (Extracellular region) Mouse mAb||50 μl||WB, E, ICC||Hu, Rt, Ms||180|
|Integrin αV (Extracellular region) Mouse mAb||50 μl||WB, E||Hu, Rt, Ms||125|
|Anti-Mouse Ig:HRP Donkey pAb||100 μl||WB, E||Ms|
The Integrin α family antibody sampler kit can be used to detect the expression level of Integrin α2, Integrin α5, Integrin αL, and Integrin αV. The kit includes high affinity mouse monoclonal antibodies to examine Integrin α expression levels in western blot, immunoprecipitation, and immunocytochemistry.