Catalog # HP5541


Histone H4

Rabbit Polyclonal

Application Dilution
ELISA1:1000
WB1:500

Size 100 μl

Species Reactivity Hu, Rt, Ms, Fr, Ck

MW 11 kDa

$245

Chromatin structure is regulated through the activity of core histones (H2A, H2B, H3, and H4) that form the nucleosome. Histone activity is regulated by a variety of post-translational modifications, including acetylation, phosphorylation, and methylation. Histone acetylation and methylation occur primarily at lysine (K) residues in the amino-terminal tail domain. These modifications are important for the regulation of histone deposition, transcriptional activation, DNA replication and repair. Acetylation and methylation of specific lysine residues creates docking sites for DNA repair, transcription, and chromatin regulatory proteins. Methylation of histones may be regulated by phosphorylation events at sites downstream of the N-terminal tail. In histone H4, both EGFR activation and inonizing radiation induce EGFR nuclear translocation and Histone H4 (Tyr-72) phosphorylation, which creates a docking site for Set8 methyltransferase. This promotes K20 methylation in Histone H4 leading to DNA synthesis and repair.

 

References
Chou, R.H. et al. (2014) Developmental Cell 30:224.
Jaskelioff, M. & Peterson, C.L. (2003) Nat Cell Biol. 5:395.
Histone H4 full length protein from calf thymus.

*For more information, see UniProt Accession P62803
Rabbit polyclonal, affinity-purified antibody is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

The antibody was affinity purified using Protein A chromatography. This antibody antibody detects a 10 kDa* protein on SDS-PAGE immunoblots of human PC3 and A431 cells, as well as rat PC12 cells. The antibody also detects a purified recombinant full length human histone H4 protein.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Western blot image of human PC3 cells untreated (lanes 1 & 3) or treated with alkaline phosphatase to dephosphorylate histone H4 (lanes 2 and 4). The blot was probed with rabbit polyclonals anti-Histone H4 (lanes 1 & 2) and anti-Histone H4 (Tyr-72) phospho-specific antibody (lanes 3 & 4).



This kit contains:

KIT SUMMARY