Catalog # GM3331

GAP43 (N-terminal region) Antibody

Mouse Monoclonal

Application Dilution
ELISA1:2000
ICC1:50
IHC1:400
WB1:1000

Size 100 μl

Species Reactivity Hu, Rt, Ms

MW 43 kDa

Isotype IgG1

DATASHEET  




$325

GAP43 (B-50, F1, neuromodulin) is a phosphoprotein found in the presynaptic membranes of neurons, where it may have important roles in the maintenance of long-term potentiation and synaptic plasticity. Regulation of GAP43 activity may occur through PKC phosphorylation of Ser-41, which occurs during neurotransmitter release in mature neurons. Calmodulin may be a negative regulator of GAP43, since it binds to GAP43 when Ca2+ is low and neuronal activity is minimal. GAP43 is a major component of the growth cone in axons of developing neurons. Thus, it may have roles in axon development and synaptogenesis.

 

References

Gianotti, C. et al. (1992) Neuron 8:843.
McIntosh, H. et al. (1989) Visual Neuroscience 2:583.
Skene, J.H., et al. (1986) Science 233:783.

Western blot of mouse brain lysate. The blot was probed with mouse monoclonal anti-GAP43 (N-terminal region) antibody at 1:500 (lane 1) or 1:1000 (lane 2).

Formalin fixed, citric acid treated parafin sections of E7 chick spinal cord. Sections were probed with anti-GAP43 (GM3331) then anti-mouse:HRP before detection using DAB. (Image provided by Carl Hobbs and Dr. Pat Doherty at Wolfson Centre for Age-Related Diseases, King's College London).



Clone (M333) was generated from a sequence corresponding to amino acids in the N-terminal region of the human GAP43. This sequence has high homology to rat and mouse GAP43.

*For more information, see UniProt Accession P17677
Mouse monoclonal, protein A purified antibody is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at -20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

This antibody detects a 43 kDa* protein corresponding to the molecular mass of GAP43 on SDS-PAGE immunoblots of adult mouse brain.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains:

KIT SUMMARY