Catalog # FM2461

FAK (Central region) Antibody

Mouse Monoclonal

Application Dilution
ELISA1:1000
WB1:250

Size 100 μl

Species Reactivity Hu, Rt, Ms

MW 125 kDa

Isotype IgG1

DATASHEET  




$245

Focal adhesion kinase (FAK) is a widely expressed cytoplasmic protein tyrosine kinase involved in signal transduction pathways important for cell spreading, migration and survival. Activation of FAK by integrin clustering leads to autophosphorylation at Tyr-397, which is a binding site for Src family kinases, PI3-Kinase, and PLCγ. The recruitment of Src family kinases results in the phosphorylation of tyrosine 407, 576, and 577 in the catalytic domain, and tyrosine 871 and 925 in the carboxy-terminal region of FAK. Thus, the phosphorylation of Tyr-397 is a critical step in the activation of FAK.

 

References

Parsons, J.T. et al. (2000) Oncogene 19:5606.
Cobb, B.S. et al. (1994) Mol. Cell. Biol. 14:147.
Schaller, M.D. et al. (1994) Mol. Cell. Biol. 14:1680.
Schlaepfer, D.D. et al. (1994) Nature 372:786-791.

Western blot analysis of HUVECs untreated (lanes 1 & 3) or treated with alkaline phosphatase (lanes 2 & 4). Blots were probed with mouse monoclonal anti-FAK (lanes 1 & 2) and anti-FAK (Tyr-397) (lanes 3 & 4).



Clone (M246) was generated from a recombinant protein containing amino acid in the central region of human FAK. This sequence has high homology to similar regions in rat and mouse FAK.

*For more information, see UniProt Accession Q05397
Mouse monoclonal antibody purified with protein A chromatography is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

The antibody detects a 125 kDa* protein on SDS-PAGE immunoblots of human HUVEC, A431, and mouse SYF cell lysates.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains:

KIT SUMMARY