Catalog # FM2381


Fyn (N-terminal region)

Mouse Monoclonal

Application Dilution
ELISA1:1000
WB1:250

Size 100 μl

Species Reactivity Hu, Rt, Ms

MW 59 kDa

Isotype IgG2b

$245

The Src family of tyrosine kinases include nine members in vertebrates, including c-Src, Yes, Fgr, Yrk, Fyn, Lyn, Hck, Lck, and Blk. Src-family kinases transduce signals that are involved in the control of a variety of cellular processes, including proliferation, differentiation, motility, and adhesion. Fyn is a 59 kDa member of the Src family of tyrosine kinases that conatins a conserved carboxy terminus and unique N-terminal region. Fyn is N-myristoylated and targeted to the plasma membrane, where it is palmitoylated. Phosphorylation regulates the activity of Fyn at conserved residues, Tyr-420 and Tyr-531. Fyn clusters in caveolae-like membrane microdomains and can interact with a variety of other signaling molecules. Fyn activity is important during T cell receptor signaling, neuronal synaptic transmission, and adhesion-mediated signaling.

 

References
Resh, M.D. (1998) Int J Biochem Cell Biol 30:1159.
Thomas, S.M. & Brugge, J.S. (1997) Annu Rev Cell Dev Biol 13:513.
Hunter, T. (1987) Cell 49:1.
Clone M238 was generated from a recombinant human Fyn protein that included amino acids in the N-terminal region. This sequence is highly conserved in rat and mouse Fyn.

*For more information, see UniProt Accession P06241
Mouse monoclonal antibody purified with protein A chromatography is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

The antibody detects a 59 kDa* band corresponding to Fyn on SDS-PAGE immunoblots of adult mouse brain tissue.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Western blot analysis of adult mouse brain tissue lysate. The blot was probed with mouse monoclonal anti-Fyn (N-terminal region) at 1:125 (lane 1) and 1:500 (lane 2).



This kit contains:

KIT SUMMARY