Catalog # FM0141


Fibronectin

Mouse Monoclonal

Application Dilution
ELISA1:2000
ICC1:50
IP1:50
WB1:1000

Size 100 μl

Species Reactivity Hu, Rt, Ms, Ck

MW 250 kDa

Isotype IgG1

$295

In the extracellular matrix, fibronectin provides essential connections to cells through interaction with integrins and other receptors that regulate cell adhesion, migration, and differentiation. Fibronectin is secreted as a large dimeric glycoprotein with subunits that range in size from 230 kDa to 270 kDa. Fibronectin is composed of three different types of modules termed type I, II, and III repeats, as well as two fibrin binding and two heparin binding domains, a collagen interaction region and cell attachment domain. The diverse set of binding domains provides fibronectin with the ability to interact simultaneously with other fibronectin molecules, other ECM components (e.g., collagens and proteoglycans), cell surface receptors, and extracellular enzymes. Plasma fibronectin (soluble dimeric form) is secreted by hepatocytes, while cellular fibronectin (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Fibronectin fibrilogenesis has important functions during tissue development, and during pathological progression of tissues and wound healing.

 

References
Zollinger AJ & Smith ML (2017) Matrix Biol. 60-61:27.
Wang Y & Ni H. (2016) Cell Mol Life Sci. 73(17):3265.
Schwarzbauer J & DeSimone D (2011) CSH Persp Biol. 1;3(7).
Clone (M014) was generated from human Fibronectin purified from human plasma.

*For more information, see UniProt Accession P02751
Mouse monoclonal antibody purified with protein A chromatography is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

Clone M014 mouse monoclonal antibody detects a 250 kDa* protein on SDS-PAGE immunoblots of denatured and native human A549, MeWo, and MDA-MB-231 cells, as well as human plasma fibronectin. The antibody also works for immunoprecipitation, immunohistochemistry, and immunocytochemistry, as well as binds fibronectin in live cells and unfixed extracellular matrix.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Western blot image of native human cell lysates: A549 (lanes 1 & 5), MeWo (lanes 2 & 6), and MDA-MB-231 (lanes 3 & 7), as well as human plasma Fibronectin (lanes 4 & 8). The blot was probed with mouse monoclonal antibodies anti-fibronectin FM0141 (lanes 1-4) or anti-fibronectin FM0131 (lanes 5-8) at 1:1000.

Immunocytochemical labeling of fibronectin in the extracellular matrix and cells of paraformaldehyde fixed human A549 cells. The cells were labeled with mouse monoclonal anti-fibronectin (clone M014). The antibody was detected using goat anti-mouse Ig DyLight® 594.



This kit contains:

KIT SUMMARY