Catalog # EM5161


ERK2 (C-terminal region)

Mouse Monoclonal

Application Dilution
FC1:200
ICC1:200
IP1:100
WB1:1000

Size 100 μl

Species Reactivity Hu, Rt, Ms, Ck, F

MW 42 kDa

Isotype IgG1

$245

Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs such as cell proliferation, differentiation, motility, and death. The ERK1/2 (p44/42) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines. Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK), a MAP kinase kinase (MAPKK), and a MAP kinase (MAPK). Multiple ERK1/2 MAPKKKs have been identified, including members of the Raf family as well as Mos and Tpl2/Cot. MEK1 and MEK2 are the primary MAPKKs in this pathway. MEK1 and MEK2 activate ERK1 and ERK2 through phosphorylation of activation loop residues Thr-202/Tyr-204 and Thr-185/Tyr-187, respectively. ERK1/2 are negatively regulated by a family of dual-specificity (Thr/Tyr) MAPK phosphatases. Several downstream targets of ERK1/2 have been identified, including p90RSK and the transcription factor Elk-1.

 

References

Owens, D.M. & Keyse, S.M. (2007) Oncogene 26:3203.
Murphy, L.O. & Blenis, J. (2006) Trends Biochem Sci 31:268.
Roux, P.P. & Blenis, J. (2004) Microbio Mol Biol Rev 68:320.

Clone M516 was generated from a recombinant human ERK2 protein that included amino acids residues in the C-terminal region. This sequence is conserved in rat and mouse ERK2, and has significant homology to ERK1, but not other ERK family members.

*For more information, see UniProt Accession P28482
Mouse monoclonal antibody purified with protein A chromatography is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

The antibody detects a 42 kDa* protein corresponding to ERK2 on SDS-PAGE immunoblots of human A431 epithelial cells and Jurkat T-cells. This clone may cross-react with ERK1, but not other ERK family members.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Western blot analysis of human A431 epithelial (lanes 1 & 2) and Jurkat (lanes 3 & 4) cells. The blots were probed with mouse monoclonal anti-ERK2 (C-terminal region) at 1:500 (lanes 1 & 3) or 1:2000 (lanes 2 & 4).

Immunocytochemical labeling of ERK2 in methanol and acetone fixed human NCI-H1915 lung carcinoma cells. The cells were labeled with mouse monoclonal anti-ERK2 (EM5161) antibody. The antibody was detected using appropriate secondary antibody conjugated to DyLight® 594.



This kit contains:

KIT SUMMARY