Catalog # EM1661

EGFR (Cytoplasmic) Antibody

Mouse Monoclonal

Application Dilution
ICC1:100
IP1:100
WB1:1000

Size 100 μl

Species Reactivity Hu, Rt, Ms

MW 180 kDa

Isotype IgG1

DATASHEET  




$325

The epidermal growth factor receptor (EGFR) is a transmembrane glycoprotein with an extracellular ligand-binding domain and a cytoplasmic domain with intrinsic tyrosine kinase activity. The cytoplasmic domain has a C-terminal region with multiple autophosphorylation sites (Tyr-992, 1068, 1086, 1148, and 1173). These sites are important for downstream signaling and rapid internalization. In addition, EGFR activation leads to c-Src mediated phosphorylation of Tyr-845 and Tyr-1101. The former site is required for mitogenic responses to EGFR activation, while the latter may be an SH2 binding site. Phosphorylation of EGFR on serine and threonine residues is thought to represent a mechanism for regulation of receptor kinase activity and internalization. These sites include a PKC site (Thr-654), CAMKII sites (Ser-1046, 1047, 1057, and 1142), and constitutively phosphorylated sites (Ser-967 and Ser-1002). Thus, the regulation of EGFR activity involves a complex series of phosphorylation events at multiple sites throughout the intracellular portion of the receptor.

 

References
Morandell, S. et al. (2008) Proteomics. 8(21):4383.
Boeri Erba, E. et al. (2005) Mol. Cell. Prot. 4:1107.
Carpenter, G. (2000) Bioessays 22:697.

Western blot image of human A431 cells untreated (lanes 1 & 3) or treated with EGF (100 ng/ml) for 5 min. The blot was probed with anti-EGFR (Tyr-1101) monoclonal antibody (lanes 1 & 2) or antiEGFR (Cytoplasmic) monoclonal antibody (lanes 3 & 4).

Immunocytochemical labeling in A431 cells untreated or treated with EGF (100 ng/ml) for 5 min. The cells were labeled with mouse monoclonals anti-EGFR (EM1661) and anti-EGFR (Tyr-1101) (EM1991).



Clone M166 was generated from a synthetic peptide (coupled to KLH) corresponding to amino acid residues in the cytoplasmic region of human EGFR (ErbB-1). This human EGFR sequence has high homology to rat and mouse EGFR, and is not conserved in other ErbB family members.

*For more information, see UniProt Accession P00533
Mouse monoclonal, protein A purified antibody is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at -20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

This antibody antibody detects a 180 kDa* protein on SDS-PAGE immunoblots of human A431 cells. The antibody can be used for immunocytochemical labeling and immunoprecipitation assays.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains:

KIT SUMMARY