Catalog # EK6680

EB1/2/3 Antibody Kit

Antibody Sampler Kit

DATASHEET  




$395

Microtubles (MTs) are oriented with a fast growing plus-end and a slower growing minus-end. The MT plus-end is a crucial site for the regulation of MT dynamics and MT association with different cellular organelles by several groups of plus-end tracking proteins (+TIPs). These +TIPs form comet-like accumulations at the plus ends of MTs to regulate MT dynamics and interactions. The +TIPs include diverse groups of proteins, such as motor and nonmotor proteins, MT polymerases and depolymerases as well as various regulatory and adaptor proteins. The End-Binding (EB) family of +TIPs includes EB1 (MAPRE1), EB2 (MAPRE2, RP1), and EB3 (MAPRE3, EBF3). EB proteins are ubiquitiously expressed +TIPs that can dimerize through a coiled-coil C-terminal region, and bind MTs through an N-terminal calponin homology domain. EB proteins can stabilize MTs, increase MT dynamics, and suppress MT pauses. These activities of EB proteins are important for mitotic spindle formatin and chromsome alighnment during mitosis, and may have important roles in the centrosome and cilia assembly.

References
Morrison, E.E. (2009) Adv Exp Med Biol. 656:41-50.
Gouveia, S.M. & Akhmanova, A. (2010) Int Rev Cell Mol Biol. 285:1-74.
Gouveia, S.M. & Akhmanova, A. (2010) Int Rev Cell Mol Biol. 285:1.
Komarova, Y. et al. (2012) Mol Cell. 48:914.

Western blot of EB proteins in mouse brain (lanes 1-5). The blot was probed with rat monoclonals EM5041 anti-EB1 (lane 1), EM5061 anti-EB1/2/3 (lane 2), EM5081 anti-EB2 (lane 3), EM5101 anti-EB3 (lane 4), and EM5091 anti-EB3 (lane 5). Then the antibodies were detected using goat anti-Rat IgG Light Chain specific:HRP (RS3121).

Immunocytochemical labeling of EB1 in paraformaldehyde-fixed and NP40-permeabilized A431 cells. The cells were dual labeled with mouse monoclonal anti-α-Tubulin (TM4111) (left) and rat monoclonal anti-EB1 (EM5041) (middle). The antibodies were detected using either goat anti-mouse:DyLight® 488 (MS3011) or goat anti-Rat:DyLight® 594 (RS3111).



Rat monoclonal antibody purified with protein G chromatography is supplied in phosphate-buffered saline and 0.05% sodium azide. Aliquot and Store at –20°C. Stable for 1 year. Secondary reagent is supplied in phosphate-buffered saline (PBS), 50% glycerol, and 1 mg/ml BSA. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.



*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Product References:

EM5041 Komarova, Y. et al. (2005) Mol Biol Cell. 16(11):5334. WB, ICC: CHO-K1 cellls
EM5081 Komarova, Y. et al. (2005) Mol Biol Cell. 16(11):5334. WB, ICC: CHO-K1 cellls
EM5101 Komarova, Y. et al. (2005) Mol Biol Cell. 16(11):5334. WB, ICC: CHO-K1 cellls

This kit contains:

CATALOG#DESCRIPTIONSIZEAPPLICATIONSSPECIES REACTIVITYMW (kDa)
EB1 (C-terminal region) Rat mAb50 μlWB, E, ICCHu, Rt, Ms30
EB1/EB2/EB3 (C-terminal region) Rat mAb 50 μlWB, E, ICCHu, Rt, Ms30/32/36
EB2 Rat mAb 50 μlWB, E, ICCHu, Rt, Ms36
EB3 Rat mAb 50 μlWB, E, ICCHu, Rt, Ms32
EB3 Rat mAb 50 μlWB, E, ICCHu, Rt, Ms32
Anti-Rat IgG:HRP Donkey pAb100 μlWB, E, ICC, IHCRt
KIT SUMMARY

The end-binding (EB) protein family antibody sampler kit can be used to detect the expression of EB1, EB2, and EB3 proteins. The kit includes an anti-Rat IgG:HRP secondary reagent for detection of rat monoclonal antibody in western blot, ELISA, or immunocytochemistry.