Catalog # DM5931

Desmoglein-1 (C-terminal region) Antibody

Mouse Monoclonal

Application Dilution
ELISA1:2000
WB1:1000

Size 100 μl

Species Reactivity Hu, Rt, Ms, Ck

MW 165 kDa

Isotype IgG2b

DATASHEET  




$245

The Desmoglein family of transmembrane glycoproteins include desmoglein 1, 2, and 3. Desmoglein-1 (Dsg1, CDHF4) is found in specialized plasma membrane junctions known as desmosome junctions. These junctions serve as membrane anchorage domains for intermediate filaments and microfilaments. Actin microfilaments anchor at adherens junctions to form an array of junction sizes, shapes, and positions. These junctions generally contain E- or N-Cadherins whose cytoplasmic regions are associated with cytoplasmic proteins such as α- and β- catenins, vinculin, α-actinin, and radixin. Intermediate filaments (IFs) are anchored at desmosomes by the desmosome-specific cadherin family members, desmocollin and desmoglein. These proteins associate with cytoplasmic proteins such as desmoplakin and plakoglobin (γ-Catenin). Plakoglobin is the only junctional protein found at both desmosomes and adherens junctions and, therefore, plays a crucial role in cell adhesion.

 

References
Mathur, M, et al. (1994) J Biol Chem. 269(19):14075.
Wheeler, GN, et al. (1991) PNAS. 88(11):4796.

Western blot analysis of human A431 (lane 1) and human MDA-MB-231 (lane 2) cell lysates. The blots were probed with mouse monoclonal anti-Desmoglein-1 (DM5931) at a dilution of 1:1000.



Clone M593 was generated from a recombinant protein containing amino acid residues in the c-terminal region of human desmoglein 1. This sequence has high homology with similar regions in rat and mouse desmoglein 1.

*For more information, see UniProt Accession Q02413
Mouse monoclonal purified with protein A chromatography is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

Clone M593 was purified using Protein A chromatography. The antibody detects a 165 kDa* protein corresponding to the molecular mass of Desmoglein 1 on SDS-PAGE immunoblots of human A431, HeLa, and MDA-MB-231 cells.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains:

KIT SUMMARY