Catalog # DM4921

D4-GDI, cleavage-specific Antibody

Mouse Monoclonal

Application Dilution
FC1:1000
IHC1:100
IP1:250
WB1:1000

Size 100 μl

Species Reactivity Hu, Rt, Ms

MW 28 kDa

Isotype IgG2b

DATASHEET  




$245

D4-GDI (GDP dissociation inhibitor) is a negative regulator of the ras-related Rho family of GTPases. Since the Rho GTPases promote cytoskeletal and membrane changes associated with apoptotic cell death, the removal of the D4-GDI block through its cleavage is important for inducing apoptosis. Caspase-3 cleaves the 28 kD mature form of D4-GDI to give a 23 kD and 5 kD size fragment. The 23 kD fragment then translocates to the nucleus. The mechanisms involving cleavage of D4-GDI with apoptosis are not presently known. Activation of the Jun N-terminal kinase, a regulator of apoptosis, may be one of the mechanisms.

 

References

Krieser, R.J. et al. (1999) Cell Death Differ. 6:412.
Na, S. et al. (1996) J Biol Chem. 271:11209.

Western blot analysis of D4-GDI in Jurkat cells using anti-D4-GDI and anti-D4-GDI cleavage-specific antibody DM4921. The cells were treated with 2 μM staurosporine for different time periods. DM4921 specifically detects only the 23 kDa cleaved form of the protein.



Clone 97A1015 was developed from a synthetic peptide corresponding to the region of the Fas-induced cleavage site of human D4-GDI. The sequence is well conserved in rat and mouse D4-GDI.

*For more information, see UniProt Accession P52566
Mouse monoclonal antibody purified with protein G chromatography is supplied in 100µl phosphate-buffered saline, 0.05% BSA, and 0.05% sodium azide. Store at 4°C. For long term storage, store at –20°C.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

The antibody detects cleaved D4-GDI at 23 kDa* in Jurkat cells treated with staurosporine. The antibody does not detect full length D4-GDI. DM4921 can be used for western blot, flow cytometry, immunoprecipitation, and immunohistochemistry.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains:

KIT SUMMARY