Catalog # DM4921
D4-GDI, cleavage-specific
Mouse Monoclonal
| FC | 1:1000 |
| IHC | 1:100 |
| IP | 1:250 |
| WB | 1:1000 |
Size 100 μl
Species Reactivity Hu, Rt, Ms
MW 28 kDa
Isotype IgG2b
D4-GDI (GDP dissociation inhibitor) is a negative regulator of the ras-related Rho family of GTPases. Since the Rho GTPases promote cytoskeletal and membrane changes associated with apoptotic cell death, the removal of the D4-GDI block through its cleavage is important for inducing apoptosis. Caspase-3 cleaves the 28 kD mature form of D4-GDI to give a 23 kD and 5 kD size fragment. The 23 kD fragment then translocates to the nucleus. The mechanisms involving cleavage of D4-GDI with apoptosis are not presently known. Activation of the Jun N-terminal kinase, a regulator of apoptosis, may be one of the mechanisms.
References
Krieser, R.J. et al. (1999) Cell Death Differ. 6:412.
Na, S. et al. (1996) J Biol Chem. 271:11209.
*For more information, see UniProt Accession P52566
The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.
*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
Western blot analysis of D4-GDI in Jurkat cells using anti-D4-GDI and anti-D4-GDI cleavage-specific antibody DM4921. The cells were treated with 2 μM staurosporine for different time periods. DM4921 specifically detects only the 23 kDa cleaved form of the protein.
This kit contains:
