Catalog # DM2051

β-Dystroglycan (C-terminal region) Antibody

Mouse Monoclonal

Application Dilution
ELISA1:2000
WB1:1000

Size 100 μl

Species Reactivity Hu

MW 43 kDa

Isotype IgG2a

DATASHEET  




$245

Dystroglycans are essential elements of the neuromuscular junction (NMJ). The gene for dystroglycan is expressed as a precursor protein that is post-translationally cleaved into a 156 kDa extracellular peripheral membrane protein called α-dystroglycan and a 43 kDa transmembrane protein, β-Dystroglycan. The latter protein contains a PPxY motif that promotes binding to WW domain-containing proteins, such as utrophin and dystrophin. Phosphorylation at tyrosine 892 within the PPxY motif may regulate c-Src interactions with β-Dystroglycan, as well as inhibit interactions with WW domain proteins. In skeletal muscle, β-Dystroglycan is normally localized to the plasma membrane, however phosphorylation of Tyr-892 leads to localization of β-Dystroglycan to endosomal compartments along with c-Src. Thus, phosphorylation at Tyr-892 may have important roles in altering the localization of β-Dystroglycan during NMJ formation.

 

References
James, M. et al. (2000) J Cell Sci. 113(Pt 10):1717-1726.
Sotgia, F. et al. (2003) Biochem. 42(23):7110-7123.

Western blot analysis of HepG2 cells untreated (lanes 1 & 3) or treated with pervanadate (1 mM) for 30 min (lanes 2 & 4). Blots were probed with anti-β-Dystroglycan (lanes 1 & 2) and anti-β-Dystroglycan (Tyr-892) (lanes 3 & 4).



Clone (M205) was generated from a recombinant protein corresponding to the C-terminal region of human β-Dystroglycan. This sequence has high homology to the conserved region in mouse, cow, and rabbit β-Dystroglycan.

*For more information, see UniProt Accession Q14118
Mouse monoclonal antibody purified with protein A chromatography is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

The antibody detects a 43 kDa* protein on SDS-PAGE immunoblots of human HepG2, Hct116, and HeLa cells.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains:

KIT SUMMARY