Catalog # CP3621

δ1-Catenin (Thr-916), phospho-specific Antibody

Rabbit Polyclonal

Application Dilution
ELISA1:2000
ICC1:50
WB1:1000

Size 100 μl

Species Reactivity Hu, Rt, Ms

MW 110 kDa

DATASHEET  




$295

Catenins have emerged as molecular sensors that integrate cell-cell junctions and cytoskeletal dynamics with signaling pathways that control morphogenesis and cell to cell communication. δ1-Catenin (p120 catenin) is a catenin family member which contains an N-terminal coiled-coil domain, a regulatory domain containing multiple phosphorylation sites, and a central Armadillo repeat domain. δ1-Catenin regulates E-cadherin turnover, and has both positive and negative effects on cadherin-mediated adhesion. Actin dynamics are also regulated by δ1-Catenin, which can modulate RhoA, Rac and cdc42 activity. δ1-Catenin is phosphorylated at multiple tyrosine, serine and threonine sites both in vitro and in vivo. High levels of δ1-Catenin phosphorylated at Tyr-228 are commonly seen in several carcinoma cell lines and after EGFR activation. Many other tyrosine sites are also phosphorylated in the N-terminal region including Tyr-96, Tyr-112, Tyr-280, and Tyr-302. In addition, Thr-310 and Thr-916 are constituitively phosphorylated in many cell types, however this phosphorylation may occur only in δ1-Catenin associated with the plasma membrane.

 

References

Fukumoto, Y. et al. (2008) Exp. Cell Res. 314:52.
Reynolds, A.B. & Roczniak-Ferguson, A. (2004) Oncogene 23:7947.
Mariner, D.J. et al. (2001) J. Biol. Chem. 276:28006.

Western blot analysis of δ1-Catenin phosphorylation in A431 cells stimulated with calyculin A (100 nM) for 30 min. (lanes 1 & 3) then the blot was treated with lambda phosphatase (lanes 2 & 4). The blots were probed with either mouse monoclonal anti-δ1-Catenin (a.a. 275-285) (lanes 1 & 2) or rabbit polyclonal anti-δ1-Catenin (Thr-916) (lanes 3 & 4).

Immunocytochemical labeling of δ1-Catenin in control (Top row) or calyculin A-treated A431 cells (Bottom row). The cells were labeled with rabbit polyclonal δ1-Catenin (Thr-916) (Left) or mouse monoclonal δ1-Catenin (a.a. 275-285) (Middle). Demonstration of the overlapping labeling patterns is shown on the right. The antibodies were detected using goat anti-rabbit DyLight® 488 or goat anti-mouse-DyLight® 594.



Phospho-δ1-Catenin (Thr-916) synthetic peptide corresponds to amino acid residues around threonine 916 of human δ1-Catenin. This peptide sequence is highly conserved in rat and mouse δ1-Catenin.

*For more information, see UniProt Accession O60716
Rabbit polyclonal, affinity-purified antibody is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

This antibody was affinity purified using phospho-δ1-Catenin (Thr-916) peptide (without carrier). The antibody detects a 110 kDa* protein corresponding to the molecular mass of δ1-Catenin on SDS-PAGE immunoblots of A431 cells treated with calyculin A, but does not detect this band after lambda phosphatase treatment.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains:

KIT SUMMARY