Catalog # CM4891

Caspase-1 (C-terminal region) Antibody

Mouse Monoclonal

Application Dilution
IHC1:100
WB1:1000

Size 100 μl

Species Reactivity Hu, Rt, Ms

MW 45 kDa

Isotype IgG1

DATASHEET  




$245

Caspase proteins are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis (1). The precursor form of all caspase proteins is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into tetramer to form the active enzyme. Caspase-1 (ICE, IL-1b converting enzyme) is similar to the cell death gene CED-3 of Caenorhabditilis elegans and regulates multiple proinflammatory cytokines, including interleukin-1b and interferon-gamma-inducing factor.

 

References
Salvesen, G.S. & Riedl SJ. (2008) Adv Exp Med Biol. 615:13.
Adams, J.M. & Cory S. (2002) Curr Opin Cell Biol. 14(6):715.
Shi Y. (2002) Mol Cell. 9(3):459.

Western blot analysis of Caspase-1 using anti-Caspase-1 monoclonal antibody (CM4891) at 1:1000 to probe blots containing human HeLa (A) and mouse NIH3T3 (B) lysates.



Clone 14F468 was generated from a synthetic peptide corresponding to amino acids 371-390 (RKVRFSFEQPDGRAQMPTTE) in the C-terminal region of human caspase-1. This sequence has high homology to the conserved region in mouse and rat caspase-1.

*For more information, see UniProt Accession P29466
Mouse monoclonal antibody purified with protein G chromatography is supplied in 100µl phosphate-buffered saline, 0.05% BSA, and 0.05% sodium azide. Store at 4°C. For long term storage, store at –20°C.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

The antibody detects pro Caspase-1 at 45 kDa* and the cleaved forms of Caspase-1 that contain amino acid 371-390. The antibody can be used for western blot and immunohistochemistry.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains:

KIT SUMMARY