Catalog # CM4711


CREB (a.a. 123-136)

Mouse Monoclonal

Application Dilution
ELISA1:1000
WB1:500

Size 100 μl

Species Reactivity Hu, Rt, Ms, Ck, F

MW 43 kDa

Isotype IgG1

$245

CREB (cyclic AMP response element-binding protein) is a stimulus-induced transcription factor that plays pivotal roles in cell survival and proliferation. CREB is expressed in various tissues, and has important gene-regulating roles in the nervous system. The transactivation function of CREB is primarily regulated through Ser-133 phosphorylation by cAMP-dependent protein kinase A (PKA) and related kinases. CREB is phosphorylated at other sites in response to calcium influx and DNA damage. The DNA-damage responsive nuclear kinase, HIPK2, can phosphorylate Ser-271 but not Ser-133 in CREB, and this phosphorylation activates CREB transactivation function. Mutation of Ser-271 to Glu-271 potentiates the CREB transactivation function. Thus, phosphorylation of Ser-271 may be the mode of activation for CREB-dependent transcription in response to genotoxic stress.

 

References
Lui, C. et al. (2012) IUBMB Life. 64(3):209.
Sakamoto, K. et al. (2010) Mol. Biol. Cell 21:2966.
Gouveia, S.M. & Akhmanova, A. (2010) Int Rev Cell Mol Biol. 285:1-74.
Clone 82B514.2 was generated from a synthetic peptide (coupled to carrier protein) corresponding to amino acids 123 to 136 in human CREB. This sequence is well conserved in rat, mouse, chicken, and zebrafish CREB.

*For more information, see UniProt Accession P16220
Mouse monoclonal purified with protein G chromatography is supplied in 100μl phosphate-buffered saline with 0.2% gelatin and 0.05% sodium azide. Store at 4°C, stable for 6 months. For long term storage, aliquot and store at -20°C.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

The antibody detects a 43 kDa* protein corresponding to the molecular mass of CREB on SDS-PAGE immunoblots of human HeLa cells.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Western blot analysis of CREB expression in HeLa cells. The blot was probed with mouse monoclonal CREB (a.a. 123-136) antibody (CM4711) at 1:250 dilution.



This kit contains:

KIT SUMMARY