Catalog # CM2991


CaM Kinase IV (N-terminal region)

Mouse Monoclonal

Application Dilution
ELISA1:2000
ICC1:100
WB1:1000

Size 100 μl

Species Reactivity Hu, Rt, Ms

MW 60 kDa

Isotype IgG1

$245

Hormones, growth factors and other cell signals cause increases in cytosolic Ca2+, which leads to cellular responses mediated by various Ca2+-binding proteins. Calmodulin (CaM) is a primary receptor for Ca2+ in cells. CaM functions as an allosteric activator of many enzymatic proteins including CaM kinases. CaM kinase IV is an important member of the CaM kinase family that has roles in neuronal long-term potentiation and memory, as well as T-cell receptor signaling. The Ca2+/calmodulin-dependent CaMKK phosphorylates CaM kinase IV, releasing the autoinhibitory effect and thus activating the kinase. The activated CaMKIV further autophosphorylates itself at Thr-200 to render the kinase constitutively active. The threonine phosphorylation state of CaMKIV can be downregulated by PP2A dephosphorylation.

 

References
Racioppi, L. & Means, A.R. (2008) Trends Immunol. 29(12):600.
Kang, H. et al. (2001) Cell 106:771.
Kasahara, J. et al. (2001) J. Biol. Chem. 276:24044.
Clone (M299) was generated from a recombinant sequence that included amino acids in the N-terminal region of human CaM Kinase IV. This sequence has high homology to rat and mouse CaM Kinase IV.

*For more information, see UniProt Accession Q16566
Mouse monoclonal, protein A purified antibody is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at -20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

This antibody detects a 60 kDa* protein corresponding to the molecular mass of CaM Kinase IV on SDS-PAGE immunoblots of human Jurkat cells.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Western blot of human Jurkat cell lysate. The blot was probed with mouse monoclonal anti-CaM Kinase IV (N-terminal region) antibody at 1:250 (lane 1), 1:500 (lane 2), or 1:1000 (lane 3).

Immunocytochemical labeling of CaM Kinase IV in aldehyde fixed and NP-40 permeabilized human NCI-H446 lung carcinoma cells. The cells were labeled with mouse monoclonal anti-CaM Kinase IV (CM2991). The antibody was detected using goat anti-mouse DyLight® 594.



This kit contains:

KIT SUMMARY