Catalog # CM2471


Csk (N-terminal region)

Mouse Monoclonal

Application Dilution
ELISA1:2000
WB1:1000

Size 100 μl

Species Reactivity Hu, Rt, Ms

MW 50 kDa

Isotype IgG1

$245

C-terminal Src kinase (Csk) is a ubiquitously expressed tyrosine kinase that phosphorylates c-Src at Tyr-530, down-regulating Src kinase activity. In vitro, Csk can also phosphorylate other members of the Src family, such as Lck, c-Fgr, Fyn, and Lyn, at their conserved C-terminal tyrosine residue. The structure of Csk resembles c-Src and includes an SH3, SH2, and a catalytic domain. However, Csk lacks the catalytic domain autophosphorylation site, the C-terminal regulatory tyrosine and the N-terminal myristoylation signal of c-Src. Csk is involved in the regulation of integrin signaling during cell attachment and interacts with FAK and paxillin in podosomes. Csk gene knockout leads to neural tube defects and embryonic lethality in mice. In Csk-deficient mouse embryonic fibroblasts, actin stress fiber formation via G-protein signaling is completely abolished, suggesting that Csk may also play a critical role in linking G-protein signals to actin cytoskeletal reorganization.

 

References
Lowry, W.E. et al. (2002) Dev. Cell 2:733.
Bergman, M. et al. (1995) Mol. Cell. Biol. 15:711.
Sabe, H. et al. (1992) Mol. Cell. Biol. 12:4706.
Nada, S. et al. (1991) Nature 351:69.
Okada, M. et al. (1991) J. Biol. Chem. 266:24249.
Clone (M247) was generated from a recombinant human Csk protein that included amino acids residues in the N-terminal region. This region is highly conserved in rat and mouse Csk and has low homology to other kinases.

*For more information, see UniProt Accession P41240
Mouse monoclonal antibody purified with protein A chromatography is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

This antibody detects a 50 kDa* band corresponding to Csk in western blots of human Jurkat and mouse macrophage cell lysates.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Western blot image of human Jurkat cells. The blot was probed with mouse monoclonal anti-Csk (N-terminal region) at 1:250 (lane 1) and 1:1000 (lane 2).



This kit contains:

KIT SUMMARY