Catalog # CM0351


VE-Cadherin (Extracellular region)

Mouse Monoclonal

Application Dilution
ELISA1:1000
FB1:25
ICC1:100
WB1:250

Size 100 μl

Species Reactivity Hu

MW 140 kDa

Isotype IgG1

$295

Cadherins are transmembrane glycoproteins vital in calcium-dependent cell-cell adhesion during tissue differentiation. Cadherins cluster to form foci of homophilic binding units. A key determinant to the strength of the cadherin-mediated adhesion may be by the juxtamembrane region in cadherins. VE-cadherin (Cadherin 5) is the major cadherin found in endothelial cells and has important roles during angiogenesis and maintenance of barrier permeability. The cytoplasmic domain of VE-cadherin comprises the juxtamembrane domain that binds to the p120 catenin, and the carboxylterminal domain that interacts with β- or γ-catenins. Modulation of tyrosine phosphorylation on one or more of the nine tyrosine sites in the cytoplasmic domain may be important for regulating both angiogenesis and permeability. Phosphorylation of Tyr-658 and Tyr-731 alters catenin binding, restores cell migration, and decreases barrier permeability. While VEGF-induced phosphorylation of Tyr-685 occurs through c-Src, and regulates endothelial cell migration, but not permeability.

 

References

Wallez Y. et al. (2007) Oncogene 26:1067.
Baumeister U. et al. (2005) EMBOJ 24:1686.
Potter M.D. et al. (2005) J Biol. Chem. 280(36):31906.

Clone M035 was generated from a recombinant protein that included amino acids in the extracellular region of human VE-Cadherin. This sequence is not conserved in other cadherin family members.

*For more information, see UniProt Accession P33151
Mouse monoclonal antibody purified with protein A chromatography is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

Clone M035 detects a 140 kDa* band corresponding to VE-cadherin in western blots of human endothelial cells. The antibody is recommended for use in western blot, ELISA and immunocytochemsitry, as well as for function blocking VE-cadherin homotypic adhesion.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Western blot image of human umbilical vein endothelial cells stimulated with pervanadate (1 mM) for 30 min. then the blots were untreated (lanes 1 & 3) or treated with alkaline phosphatase (lanes 2 & 4). The blots were probed with rabbit polyclonal anti-VE-cadherin (Tyr-685) (lanes 1 & 2) or mouse monoclonal anti-VE-cadherin (lanes 3 & 4).

Immunocytochemical labeling of VE-Cadherin in aldehyde fixed and NP-40 permeabilized human NCI-H446 lung carcinoma cells. The cells were labeled with mouse monoclonal anti-VE-Cadherin (CM0351). The antibody was detected using goat anti-mouse DyLight® 594.



This kit contains:

KIT SUMMARY