Catalog # CK6760

Cadherin Family (E-, N-, and P-) Antibody Kit

Antibody Sampler Kit

DATASHEET  




$395

Cadherins are transmembrane glycoproteins vital in calcium-dependent cell-cell adhesion during tissue differentiation. Cadherins cluster to form foci of homophilic binding units. A key determinant to the strength of the cadherin-mediated adhesion may be by the juxtamembrane region in cadherins. This region induces clustering and also binds to the protein p120 catenin. The cytoplasmic region is highly conserved in sequence and has been shown experimentally to regulate the cell-cell binding function of the extracellular domain of E-cadherin, possibly through interaction with the cytoskeleton. Many cadherins are regulated by phosphorylation, including N-cadherin and E-cadherin. P-Cadherin (Cadherin-3) is localized in placenta while E-Cadherin (Cadherin-1) and N-Cadherin (Cadherin-2) are found in epithelial and neural tissues, respectively. P-Cadherin is expressed in normal epithelial cells and some cancer cells, and its sequence contains 5 cadherin domains in the extracellular region.

References
Takeichi, M. (1988) Development 102:639.
Xu, Y. et al. (1997) J. Biol. Chem. 272(21):13463.
Xu, Y. & Carpenter, G. (1999) J. Cell. Bioch. 75:264.
Qi, J. et al. (2006) Mol. Biol. Cell 17(3):1261.

Western blot image of human A431 cells (lanes 1-4). The blots were probed with mouse monoclonals anti-E-Cadherin (Cytoplasmic) at 1:1000 (lane 1) and 1:4000 (lane 2) and anti-E-Cadherin (C-terminal fragment) at 1:250 (lane 3) and 1:1000 (lane 4).

Western blot image of mouse brain lysate immunoprecipitated with no antibody (lane 1), anti-N-Cadherin (CP1751) rabbit polyclonal antibody (lane 2), and whole mouse brain lysate (lane 3). The blot was probed with anti-N-cadherin (Cytoplasmic) mouse monoclonal antibody (lanes 1-3) and detected using anti-Mouse Ig Light Chain specific:HRP secondary antibody.



Rabbit polyclonal and mouse monoclonal antibodies are supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store all at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.



*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Product References:

CP1751 Chang, Y.J. et al. (2014) Mol Cell Biol. 34(6):1003 WB: PC12-SH2B1β cell
CP1921 Gu, H. et al. (2014) FASEB Journal 28(10):4223. WB: human small airway epithelial cells
CP1921 SalaheldeenE. et al. (2014) J Histo Cytochem. 62(9):632. WB: seminiferous tubules
CM1681 Milara, J. et al. (2013) Thorax. 68(5):410-20. IF: human pulmonary tissue
CM1681 Vittal, R. et al. (2013) AJP Lung Cell Mol Phys 304(6):401. WB: Rat & Human Epithelial Cells
CP1751 Sato, F. et al. (2012) Intern J Molec Med 30:495. WB: Human pancreatic cancer BxPC-3 cells
CP1751 Wu, Y. et al. (2012) Int J Oncol. 41(4):1337. WB: human pancreatic cancer cells (PANC-1 PaCa-2)
CM1701 Wang, T.C. et al. (2011) J. Cell. Physiol. 226:2063. WB, ICC: rat PC12 cells
CP1751 Ferreri, D.M. et al. (2008) Cell Comm Adh. 15(4):333. WB: human, bovine VECs

This kit contains:

CATALOG#DESCRIPTIONSIZEAPPLICATIONSSPECIES REACTIVITYMW (kDa)
E-Cadherin (Cytoplasmic) Mouse mAb 50 μlWB, E, IP, ICC, IHCHu, Rt, Ms120
E-Cadherin (C-terminal fragment) Mouse mAb50 μlWB, EHu, Rt, Ms120
E-Cadherin (a.a. 774-786) Rabbit pAb 50 μlWB, EHu, Rt, Ms120
N-Cadherin (Cytoplasmic) Mouse mAb50 μlWB, E, IP, ICCHu, Rt, Ms130
N-Cadherin (a.a. 811-824) Rabbit pAb50 μlWB, E, IP, ICCHu, Rt, Ms130
P-Cadherin (N-terminal region) Mouse mAb50 μlWB, E, ICCHu, Rt, Ms120
KIT SUMMARY

The cadherin family antibody sampler kit can be used to detect the expression level of E-cadherin, N-Cadherin, and P-cadherin. The kit includes high affinity mouse monoclonal and rabbit polyclonal antibodies to examine cadherin expression levels in western blot, ELISA, immunoprecipitation, and immunocytochemistry.