Catalog # CK6760

Cadherin Family (E-, N-, and P-) Antibody Kit

Antibody Sampler Kit

Application / Dilution
ELISA, WB, IP, ICC, IHC

Species Reactivity

Hu, Rt, Ms

$395


DATASHEET  

KIT SUMMARY

The cadherin family antibody sampler kit can be used to detect the expression level of E-cadherin, N-Cadherin, and P-cadherin. The kit includes high affinity mouse monoclonal and rabbit polyclonal antibodies to examine cadherin expression levels in western blot, ELISA, immunoprecipitation, and immunocytochemistry.

Western blot image of human A431 cells (lanes 1-4). The blots were probed with mouse monoclonals anti-E-Cadherin (Cytoplasmic) at 1:1000 (lane 1) and 1:4000 (lane 2) and anti-E-Cadherin (C-terminal fragment) at 1:250 (lane 3) and 1:1000 (lane 4).

Western blot image of mouse brain lysate immunoprecipitated with no antibody (lane 1), anti-N-Cadherin (CP1751) rabbit polyclonal antibody (lane 2), and whole mouse brain lysate (lane 3). The blot was probed with anti-N-cadherin (Cytoplasmic) mouse monoclonal antibody (lanes 1-3) and detected using anti-Mouse Ig Light Chain specific:HRP secondary antibody.



Rabbit polyclonal and mouse monoclonal antibodies are supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store all at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.



*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Product References:

CP1751 Chang, Y.J. et al. (2014) Mol Cell Biol. 34(6):1003 WB: PC12-SH2B1β cell
CP1921 Gu, H. et al. (2014) FASEB Journal 28(10):4223. WB: human small airway epithelial cells
CP1921 SalaheldeenE. et al. (2014) J Histo Cytochem. 62(9):632. WB: seminiferous tubules
CM1681 Milara, J. et al. (2013) Thorax. 68(5):410-20. IF: human pulmonary tissue
CM1681 Vittal, R. et al. (2013) AJP Lung Cell Mol Phys 304(6):401. WB: Rat & Human Epithelial Cells
CP1751 Sato, F. et al. (2012) Intern J Molec Med 30:495. WB: Human pancreatic cancer BxPC-3 cells
CP1751 Wu, Y. et al. (2012) Int J Oncol. 41(4):1337. WB: human pancreatic cancer cells (PANC-1 PaCa-2)
CM1701 Wang, T.C. et al. (2011) J. Cell. Physiol. 226:2063. WB, ICC: rat PC12 cells
CP1751 Ferreri, D.M. et al. (2008) Cell Comm Adh. 15(4):333. WB: human, bovine VECs

This kit contains:

CATALOG#DESCRIPTIONSIZEAPPLICATIONSSPECIES REACTIVITYMW (kDa)
E-Cadherin (Cytoplasmic) Mouse mAb 50 μlWB, E, IP, ICC, IHCHu, Rt, Ms120
E-Cadherin (C-terminal fragment) Mouse mAb50 μlWB, EHu, Rt, Ms120
E-Cadherin (a.a. 774-786) Rabbit pAb 50 μlWB, EHu, Rt, Ms120
N-Cadherin (Cytoplasmic) Mouse mAb50 μlWB, E, IP, ICCHu, Rt, Ms130
N-Cadherin (a.a. 811-824) Rabbit pAb50 μlWB, E, IP, ICCHu, Rt, Ms130
P-Cadherin (N-terminal region) Mouse mAb50 μlWB, E, ICCHu, Rt, Ms120