Catalog # CK6740

VE-Cadherin Phospho-Regulation Antibody Kit

Antibody Sampler Kit

DATASHEET  




$395

Cadherins are transmembrane glycoproteins vital in calcium-dependent cell-cell adhesion during tissue differentiation. Cadherins cluster to form foci of homophilic binding units. A key determinant to the strength of the cadherin-mediated adhesion may be by the juxtamembrane region in cadherins. VE-cadherin (Cadherin 5) is the major cadherin found in endothelial cells and has important roles during angiogenesis and maintenance of barrier permeability. The cytoplasmic domain of VE-cadherin comprises the juxtamembrane domain that binds to the p120 catenin, and the carboxylterminal domain that interacts with β- or γ-catenins. Modulation of tyrosine phosphorylation on one or more of the nine tyrosine sites in the cytoplasmic domain may be important for regulating both angiogenesis and permeability. Phosphorylation of Tyr-658 and Tyr-731 alters catenin binding, restores cell migration, and decreases barrier permeability. While VEGF-induced phosphorylation of Tyr-685 occurs through c-Src, and regulates endothelial cell migration, but not permeability.

References
Baumeister U. et al. (2005) EMBOJ 24:1686.
Potter M.D. et al. (2005) J Biol. Chem. 280(36):31906.
Wallez Y. et al. (2007) Oncogene 26:1067.

Western blot image of human umbilical vein endothelial cells stimulated with pervanadate (1 mM) for 30 min. then the blots were untreated (lanes 1 & 3) or treated with alkaline phosphatase (lanes 2 & 4). The blots were probed with rabbit polyclonal anti-VE-cadherin (Tyr-685) (lanes 1 & 2) or mouse monoclonal anti-VE-cadherin (lanes 3 & 4).

Immunocytochemical labeling of VE-Cadherin in paraformaldehyde-fixed and NP-40-permeabilized human umbilical vein endothelial cells. The cells were labeled with rabbit polyclonal VE-Cadherin (a.a. 770-781), then the antibody was detected using appropriate secondary antibody conjugated to Cy3. Phase image (left) and fluorescent image (right).



Primary antibodies are supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. The secondary reagents are supplied in the same buffer without azide. Store all at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.



*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Product References:

CP1981 Kishor, S.K. et al.(2015) Cardiovasc Res. 108(1):171-80 WB: HUVEC
RS3251 Kawasaki, H. et al. (2013) World J Gastroenter. 19(17):2629. WB, ICC: mouse intestinal myofibroblasts
MS3001 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot
RS3251 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot
CP1981 Adam, A.P. et al. (2010) J Biol Chem. 285(10):7045. WB: HDMECs
CP1981 Bowers, S. et al. (2010) 1188:143. Cell-Cell interactions
CP1981 Cain, R. J. et al. (2010) J Cell Biol. 188(6):863. WB: HUVECs
CP1981 Lo, CW et al. (2010) Cancer Res. 71(2):424. WB: HUVECs
CP2231 Bowers, S.L. et al. (2010) 1188:143. FB: negative control in Cell-Cell contact assay

This kit contains:

CATALOG#DESCRIPTIONSIZEAPPLICATIONSSPECIES REACTIVITYMW (kDa)
VE-Cadherin (a.a. 770-781) Rabbit pAb50 μlWB, E, ICCHu, Rt, Ms140
VE-Cadherin (Extracellular region) Mouse mAb50 μlWB, E, ICC, FCHu140
VE-Cadherin (Tyr-685), phospho-specific Rabbit pAb 50 μlWB, EHu, Rt, Ms140
Anti-Mouse Ig:HRP Donkey pAb100 μlWB, EMs
Anti-Rabbit Ig Light Chain Specific:HRP Mouse mAb100 μlWB, E, ICC, IHCRb
KIT SUMMARY

The VE-cadherin phospho-regulation antibody sampler kit can be used to detect the phosphorylation of Tyr-685 relative to total expression of VE-cadherin. The kit includes a phospho-specific antibody to detect VE-cadherin Tyr-685, and mouse monoclonal and rabbit polyclonal antibodies to examine total VE-cadherin expression. The kit also includes secondary reagents for detection of primary antibodies in various applications.