Catalog # CK6180

Coronin-1B Phospho-Regulation Antibody Kit

Antibody Sampler Kit

DATASHEET  




$395

Coronins are highly-conserved F-actin binding proteins that play important roles in lamellipodial protrusion during various types of cell motility. In yeast, coronins regulate cytoskeletal changes through inhibition of Arp2/3 complex. Human coronins have been classified in three subgroups: type I (coronin-1A, -1B, -1C), type II (coronin-2A, -2B), and type III (coronin-7). These coronins have at least one large β-propeller region that mediates protein-protein interactions and type I and II coronins have coiled-coil regions involved in oligiomerization. Coronin-1B is ubiquitously expressed and localizes to the leading edge of cell protrusions in migrating fibroblasts. Both Coronin-1B and Coronin-1A interaction with Arp2/3 complex may be regulated by phosphorylation. PKC phosphorylates the N-terminus at Ser-2, and this phosphorylation reduces interactions with Arp2/3 leading to diminished cell motility.

References
Foger, N. et al. (2006) Science 313:839.
Uetrecht, A.C. & Bear, J.E. (2006) Trends Cell Biol. 16(8):421.
Cai, L. et al. (2007) Cell 128:915

Western blot analysis of human A431 cells treated with Calyculin A (100 nM) for 30 min (lanes 1 & 3) before treatment with lambda phosphatase (lanes 2 & 4). The blots were probed with anti-Coronin-1B (C-terminal region) (lanes 1 & 2) or anti-Coronin-1B (Ser-2) (lanes 3 & 4).

Immunocytochemical labeling of coronin-1B in rabbit spleen fibroblasts treated with Calyculin A. The cells were labeled with rabbit polyclonal Coronin-1B (C-terminus) and Coronin-1B (Ser-2) antibodies, then detected using appropriate secondary antibodies conjugated to Cy3. The antibodies were used in the absence (left) or presence (right) of their respective blocking peptide (CX2585 or CX2625).



Rabbit polyclonal antibodies are supplied in phosphate-buffered saline (PBS), 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. The secondary reagents are supplied in the same buffer without azide, and the peptides are supplied in PBS and 0.05% sodium azide. Store all at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.



*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Product References:

RS3251 Kawasaki, H. et al. (2013) World J Gastroenter. 19(17):2629. WB, ICC: mouse intestinal myofibroblasts
CP2621 Williams, H.C. et al. (2012) Circ Res. 111(1):56. ICC, WB: mouse carotid artery,smooth muscle
RS3251 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot

This kit contains:

CATALOG#DESCRIPTIONSIZEAPPLICATIONSSPECIES REACTIVITYMW (kDa)
Coronin-1B (C-terminus)Rabbit pAb50 μlWB, E, ICCHu, Rt, Ms60
Coronin-1B (Ser-2), phospho-specific Rabbit pAb50 μlWB, E, ICCHu, Rt, Ms60
Coronin-1B (C-terminus) Peptide 50 μlAB, E
phospho-Coronin-1B (Ser-2) Peptide50 μlAB, E
Anti-Rabbit Ig Light Chain Specific:HRP Mouse mAb100 μlWB, E, ICC, IHCRb
KIT SUMMARY

The coronin-1B phospho-regulation antibody sampler kit can be used to detect the level of phosphorylation of coronin-1B (Ser-2) relative to the total expression level of coronin-1B. The kit also includes peptides for antibody blocking experiments and a secondary reagent for antibody detection.