Catalog # AM3581

Amphiphysin (Central region) Antibody

Mouse Monoclonal

Application Dilution
ELISA1:2000
IHC1:100
WB1:1000

Size 100 μl

Species Reactivity Hu, Rt, Ms

MW 125 kDa

Isotype IgG1

DATASHEET  




$245

Amphiphysin is associated with the cytoplasmic surface of synaptic vesicles. A subset of patients with stiff man syndrome who were also affected by breast cancer are positive for autoantibodies against this protein. Alternate splicing of this gene results in two transcript variants (Amphiphysin 1 and Amphiphysin 2) encoding different isoforms. Additional splice variants have been described, but their full length sequences have not been determined. Amphiphysin interacts with several proteins important in the synaptic vesicle cycle. Amphiphysin contains a Src homology 3 (SH3) domain that binds to the C-terminal and proline-rich region of dynamin at the synapse. Microinjection of amphiphysin inhibits synaptic vesicle endocytosis. In addition, amphiphysin binds to AP-2 at the plasma membrane and to the inositol 5-phosphatase, synaptojanin.

 

References
De Camilli, P. et al. (1993) J Exp Med. 178(6):2219.
Lichte, B. et al. (1992) EMBO J. 11(7):2521.

Western blot of adult mouse brain lysate. The blot was probed with mouse monoclonal anti-Amphiphysin (Central region) antibody at 1:500 (lane 1), 1:1000 (lane 2), or 1:2000 (lane 3).



Clone M358 was generated from a sequence corresponding to amino acids in the central region of human amphiphysin. This sequence has high homology to mouse and rat amphiphysin.

*For more information, see UniProt Accession P49418
Mouse monoclonal, protein A purified antibody is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at -20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

This antibody detects a 125 kDa* protein corresponding to the molecular mass of Amphiphysin on SDS-PAGE immunoblots of adult mouse brain.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains:

KIT SUMMARY