Catalog # VK6090
VEGFR Phospho-Regulation Antibody Kit
Antibody Sampler Kit
| ELISA, WB, ICC, AB |
Species Reactivity
| Hu, Rt, Ms |
 |
KIT SUMMARY The VEGFR phospho-regulation antibody sampler kit can be used to detect the level of phosphorylation of VEGFR-2 (Tyr-801) relative to the total expression level of VEGFR-2. In addition, the anti-VEGFR-2 (Tyr-801) can be used to detect phosphorylation of the conserved sites in VEGFR-1 (Tyr-794) and VEGFR-3 (Tyr-812). The kit also includes a VEGFR-3 antibody, as well as peptides for antibody blocking experiments. |
Western blot image of GST-recombinant human VEGFR-1 (89 kDa), VEGFR-2 (110 kDa), and VEGFR-3 (86 kDa) C-terminal regions. The blots were probed with rabbit polyclonal anti-VEGFR-2 (a.a. 1304-1317), anti-VEGFR-2 (Tyr-801), and anti-VEGFR-3 (a.a. 1285-1298).
Immunocytochemical labeling of VEGFR-2 in pervanadate-treated HUVEC cells. The cells were labeled with rabbit polyclonal VEGFR-2 (a.a. 1304-1317) and VEGFR-2 (Tyr-801) antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3. Phase images are shown on the Left.
The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.
*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
Product References:
VP2921 Chen, P. et al. (2014) Proc Natl Acad Sci. 111(15):5514 WB: HUVECsVP2921 Chen, T.T. et al. (2010) J Cell Biol. 188(4):595. WB: HUVECs + VEGF
This kit contains:
| CATALOG# | DESCRIPTION | SIZE | APPLICATIONS | SPECIES REACTIVITY | MW (kDa) |
| VEGFR-2 (a.a. 1304-1317) Rabbit pAb | 50 μl | WB, E, ICC | Hu, Rt, Ms | 180 | |
| VEGFR-2 (Tyr-801)[conserved site], phospho-specific Rabbit pAb | 50 μl | WB, E, ICC | Hu, Rt, Ms | 180 | |
| VEGFR-2 (a.a. 1304-1317) Peptide | 50 μg | AB, E | |||
| phospho-VEGFR-2 (Tyr-801) Peptide | 50 μg | AB, E | |||
| VEGFR-3 (a.a. 1285-1298) Rabbit pAb | 50 μl | WB, E | Hu, Rt | 170 | |
| VEGFR-3 (a.a. 1285-1298) Peptide | 50 μg | AB, E |
