Catalog # TX4135

phospho-α6-Tubulin (Ser-165) Peptide

Blocking Peptide

Application / Dilution

Blocking	1:1000
ELISA	50 ng/well

Size 50 μg

Microtubules (MTs) are cytoskeletal elements that play an essential role in cell division and cytoplasmic organization. MTs are dynamic polymers of a/β-Tubulin heterodimers. At least two populations of MTs, called dynamic and stable according to their rates of turnover, are readily distinguishable in cells. The proteins associated with MTs (MAPs) are among the best-known factors that regulate MT dynamics and stability. In addition, a variety of different post-translational modifications may also regulate MT dynamics and stability. Phosphorylation is one of these modifications and it can occur on serine, threonine, and tyrosine residues in α- and β-Tubulin isoforms. Multiple kinases can phosphorylate Ser-444 at the C-terminus of βIII-Tubulin in vitro, and unphosphorylated Ser-444 may be an early marker for cells of neuronal lineage. Cdk1 can phosphorylate Ser-172 in β-Tubulin during mitosis and this may impair tubulin incorporation into microtubules. In α-tubulin, PKC can phosphorylate Ser-165 leading to increased cell motility in human breast cells.

References

Abeyweera, T.P. et al. (2009) J Biol Chem. 284(26):17648.
Fourest-Lieuvin, A. et al. (2006) Mol. Biol. Cell. 17(3):1041.
Westermann, S. & Weber, K. (2003) Nat. Rev. Mol. Cell. Biol. 4:938.
Diaz-Nido, J. et al. (1990) J Biol. Chem. 265(23):13949.

Phospho-α6-Tubulin (Ser-165) synthetic peptide corresponds to amino acid residues around serine 165 of human α6-Tubulin. This sequence is well conserved in most α-tubulin isoforms from most eukaryotic species, but is not conserved in β-Tubulin.

*For more information, see UniProt Accession Q9BQE3

Blocking Peptide is supplied in 50µl phosphate-buffered saline and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

The peptide is specifically recognized by anti-α6-Tubulin (Ser-165) phospho-specific antibody (TP4131) in ELISA, and has been shown to block the reactivity of TP4131 during Western blot. In addition, the peptide is recommended for use in blocking TP4131 reactivity in immunocytochemistry.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains: