Catalog # AK6390
Arp2/3 Complex Regulation Antibody Kit
Antibody Sampler Kit
| ELISA, WB, IP, ICC |
Species Reactivity
| Hu, Rt, Ms, Ck, Fr |
 |
KIT SUMMARY The Arp2/3 complex regulation antibody sampler kit can be used to examine phosphorylation of the Arp2/3 complex subunit Arp2, as well as the Arp2/3 complex regulators N-WASP/WASP and Coronin-1B. The kit contains antibodies to monitor total expression levels of each of these proteins relative to site-specific phosphorylation. |
Western blot of human A431 cells treated with Calyculin A (100 nM) for 30 min. Blot lanes were untreated (lanes 1 & 3) or treated with lambda phosphatase (lanes 2 & 4) then probed with anti-Arp2 (C-terminal) (lanes 1 & 2) or anti-Arp2 (T237/T238) (lanes 3 & 4).
Immunocytochemical labeling of Arp2 phosphorylation in rat PC12 cells differentiated with NGF. The cells were probed with Arp2 (C-terminal region) and Arp2 (Thr-237/Thr-238) rabbit polyclonal antibodies, then the antibodies were detected using appropriate secondary antibody conjugated to Cy3.
The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.
*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
Product References:
AP3861 Osma-Garcia, I.C. et al. (2015) Eur J Immunol. doi: 10.1002 WB: mouse macrophagesAP3871 Osma-Garcia, I.C. et al. (2015) Eur J Immunol. doi: 10.1002 WB: mouse macrophages
WP2601 Uenishi, E. et al. (2013) J of Bio Chem 288(36): 25851. WB: MIN6-K8 β-cells
AP3861 Spillane, M. et al. (2012) J Neurosci. 32(49):17671. ICC: Chicken embryo neuron
CP2621 Williams, H.C. et al. (2012) Circ Res. 111(1):56. ICC, WB: mouse carotid artery,smooth muscle
AP3861 Kalwa, H. & Michel, T. (2011) J Biol Chem. 286(3):2320. WB: bovine aortic endothelial cells
AP3861 Spillane, M. et al. (2011) Dev Neurobiol. 71(9):747. ICC: chick dorsal root ganglia
AP3871 Kalwa, H. & Michel, T. (2011) J Biol Chem. 286(3):2320. WB: bovine aortic endothelial cells
WP2101 Cheney, L. et al. (2011) J Infectious Diseases. 203:1824. WB: mouse adipocytes
WP2601 Cheney, L. et al. (2011) J Infectious Diseases. 203:1824. WB: mouse adipocytes
WP2601 Pichot, C.S. et al. (2009) British J Cancer. 1 –10. WB: breast cancer cell lines
This kit contains:
| CATALOG# | DESCRIPTION | SIZE | APPLICATIONS | SPECIES REACTIVITY | MW (kDa) |
| Arp2 (C-terminal region) Rabbit pAb | 50 μl | WB, E, IP, ICC | Hu, Rt, Ms, Ck, Fr | 44 | |
| Arp2 (Thr-237/Thr-238), phospho-specific Rabbit pAb | 50 μl | WB, E, ICC | Hu, Rt, Ms, Ck | 44 | |
| Coronin-1B (C-terminus) Rabbit pAb | 50 μl | WB, E, ICC | Hu, Rt, Ms | 60 | |
| Coronin-1B (Ser-2), phospho-specific Rabbit pAb | 50 μl | WB, E, ICC | Hu, Rt, Ms | 60 | |
| WASP/N-WASP Rabbit pAb | 50 μl | WB, E, ICC | Hu, Rt, Ms | 63 | |
| N-WASP (Tyr-256), phospho-specific Rabbit pAb | 50 μl | WB, E, ICC | Hu, Rt, Ms | 65 |
