Catalog # SK6030
Stat Phospho-Regulation Antibody Kit
Antibody Sampler Kit
| ELISA, WB, IP, ICC |
Species Reactivity
| Hu, Rt, Ms |
 |
KIT SUMMARY The Stat phospho-regulation antibody sampler kit can be used to examine the level of phosphorylation of Stat1 and Stat5, as well as the total expression level of Stat1, Stat3, and Stat5. |
Western blot analysis of human A431 cells untreated (lanes 1, 3, 5, 7 & 9) or treated with EGF (100 nM) for 60 min (lanes 2, 4, 6, 8 & 10). The blots were probed with anti-Stat1 (lanes 1 & 2), anti-Stat1 (Tyr-701) (lanes 3 & 4), anti-Stat3 (lanes 5 & 6), anti-Stat5 (lanes 7 & 8), and anti-Stat5 (Tyr-694) (lanes 9 & 10).
Immunocytochemical labeling of Stat5 in control and pervanadate-treated A431 cells. The cells were labeled with mouse monoclonal Stat5 (SM2511) or Stat5 (Tyr-694) (SM1481) antibodies, then the antibodies were detected using appropriate secondary antibody conjugated to DyLight® 594.
The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.
*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
This kit contains:
| CATALOG# | DESCRIPTION | SIZE | APPLICATIONS | SPECIES REACTIVITY | MW (kDa) |
| Stat1 Mouse mAb | 50 μl | WB, E, IP, ICC | Hu, Rt, Ms | 91 | |
| Stat1 (Tyr-701), phospho-specific Mouse mAb | 50 μl | WB, E | Hu, Rt, Ms | 91 | |
| Stat3 (N-terminal region) Mouse mAb | 50 μl | WB, E, IP | Hu, Rt, Ms | 86 | |
| Stat5 (C-terminal region) Mouse mAb | 50 μl | WB, E, ICC | Hu, Rt, Ms | 92 | |
| Stat5 (Tyr-694), phospho-specific Mouse mAb | 50 μl | WB, E, ICC | Hu, Rt, Ms | 92 |
