Catalog # AX1005

phospho-Akt (Thr-34) Peptide

Blocking Peptide

Application / Dilution
ELISA50 ng/well

Size 50 μg



Akt (PKB, Rac kinase) is a 60kDa ser/thr kinase critical for controlling diverse cellular functions, including glucose metabolism, gene transcription, cell proliferation, and apoptosis. Akt phosphorylates a number of substrates including MBP, glycogen synthetase, PKA RII subunit, and histone H1. Akt is activated in response to insulin and growth factors in a PI3-kinase dependent manner. Activation of PI3-Kinase generates phosphatidylinositol 3,4-bisphosphate, which induces membrane translocation of Akt coincident with its phosphorylation at Thr-308 and Ser-473. Upon activation, Akt associates with members of the PKC family of kinases, such as PKCδ and PKCζ. Ceramide-activated PKCζ leads to phosphorylation of Thr-34 within the pleckstrin homology domain of Akt. This phosphorylation inhibits PIP3 binding to Akt preventing activation of the kinase and may lead to cermide-induced cell death.



Powell, D.J. et al. (2003) Mol. Cell Biol. 23:7794-7808.
Marte, B. & Downward. J. (1997) TIBS. 22:355-358.
Jones, P.F. et al. (1991) Proc. Natl. Acad. Sci. USA 88:4171-4175.

Phospho-Akt (Thr-34) synthetic peptide corresponds to amino acid residues around threonine 34 of human Akt. This sequence is highly conserved in rat and mouse Akt.

*For more information, see UniProt Accession P31749
Blocking Peptide is supplied in 50µl phosphate-buffered saline and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

The peptide is specifically recognized by anti-Akt (Thr-34) phospho-specific antibody (AP1001) in ELISA, and is recommended for use in blocking AP1271 reactivity in Western blot and immunocytochemistry.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains: