Catalog # GM1321

GSK-3α/β (Tyr-279/Tyr-216), phospho-specific Antibody

Mouse Monoclonal

Application / Dilution

ELISA	1:2000
WB	1:1000

Size 100 μl

Species Reactivity Hu, Rt, Ms

MW 50 kDa

Isotype IgG1

Glycogen synthase kinase-3 (GSK-3) has been implicated in fundamental cell processes such as cell fate determination, metabolism, transcriptional control, and oncogenesis. Two GSK-3 genes (α and β) have been cloned in mammals and these kinase homologues show strong sequence conservation within their catalytic domain. GSK-3β plays a critical role in cell survival by phosphorylating nuclear factor-κB (NF-κB) p65 subunit, leading to NF-κB transactivation in hepatocytes. Phosphorylation regulates the activity of both GSK-3 genes. MEK1/2 can phosphorylate tyrosine 216 (tyrosine 279 in GSK-3α), which stimulates GSK-3 kinase activity. Tyr-216 phosphorylation is required for GSK-mediated down-regulation of β-catenin activity. Also, TRAIL stimulation can increase Tyr-216 phosphorylation, and GSK-3β activity may suppress TRAIL-induced apoptosis. Inactiviation of GSK-3 occurs through Akt phosphorylation of serine 9 of GSK-3β (Serine 21 in GSK-3α). This phosphorylation may be involved in later phases of neuronal apoptosis.

References

Hagen, T. et al. (2002) J Biol Chem. 277(26):23330.
Xinbo, L. et al. (2003) Mol Cancer Ther. 2:1215.
Takahashi-Yanaga, F. et al. (2004) Bioch Biophys Res Com. 316(2):411.

Western blot analysis of rabbit spleen fibroblasts serum starved for 2 hrs (lanes 1 & 3) or treated with pervanadate (1 mM) for 30 minutes (lanes 2 & 4). The blot was probed with anti-GSK-3β (lanes 1 & 2) or anti-GSK-3α/β (Y279/Y216) (lanes 3 & 4).

Clone M132 was generated from a phospho-GSK-3β (Tyr-216) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 216 of human GSK-3β. This peptide sequence is also found in GSK-3α (Tyr-279) and is highly conserved in GSK-3 genes in rat and mouse.

*For more information, see UniProt Accession P49841

Mouse monoclonal purified with protein A chromatography is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

This mouse monoclonal antibody was purified with protein A chromatography. The antibody detects 46/50 kDa* proteins corresponding to the apparent molecular mass of GSK-3β and GSK-3α on SDS-PAGE immunoblots of pervanadate treated rabbit fibroblasts, as well as treated human SKN-SH and A431 cells.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Product References:

Sui Y. et al. (2019) Brain Res. 1719:1. (WB: APP/PS1 double-transgenic mice)
Zhu, C. et al. (2017) Mol Neurobiol. 54(10):7964. (WB: mouse hippocampal neurons)
Li, R. et al. (2015) Neuroscience. 21(294): 14. (WB: rat primary neurons)
Gloggin, S. L. et al. (2014) Toxicology reports 1: 5443. (WB: mouse mFC and HPC)
Azkona, G. et al. (2014) Neuropharmacology. 79:726. (WB: rat brain)
Ding, Y. et al. (2010) Neurobiol Disease. 39(2):156. (WB: mouse cerebral cortex)
Turnquist, H.R. et al. (2010) Blood. 115(23):4758. (WB: mouse dendrite cells)
Ding, Y. et al. (2008) J Neurosci. 28(45):11622. (WB: mouse cortex, hippocampus)

This kit contains: