Catalog # IK6750

Integrin α family Antibody Kit

Antibody Sampler Kit



Integrins are cell adhesion molecules that can mediate bidirectional transfer of signals across the plasma membrane. The cytoplasmic domains of integrin family members interact with components of the signal transduction apparatus within cells. Integrin receptors contain noncovalently associated α and β subunits that consist of a large extracellular region (the ligand-binding domain), a short transmembrane region, and a cytoplasmic domain of varying length. In mammals, at least 17 α subunits and 8 β subunits have been identified and these proteins can heterodimerize to form at least 22 different receptors. Integrin α5 and integrin β1 form a receptor for fibronectin and fibrinogen. This receptor is important for vascular development. Both integrin subunits of the fibronectin receptor are heavily glycosylated in their extracellular domains. This modification is essential for proper cell attachment to basal membranes. The integrin β3 subunit associates with integrin α2 in platelets where this glycoprotein complex acts as a fibrinogen receptor and mediates platelet aggregation. Integrin α5 has been reported to suppress apoptosis by a Bcl-2 pathway and the C-terminal region is critical for cell motility and cytoskeletal rearrangements. The integrin β2 subunit associates with integrin αL to form a receptor for ICAM family members, while integrin β3 complexes with the integrin αV subunit to form the vitronectin receptor in endothelial cells.

Wang, L. et al. (2012) J Cell Physiol. 227(2):474.
Iwamoto, D. & Calderwood, D. (2015) Cur Opin Cel Bio. 36:41.
Iwamoto, D, Calderwood, D.(2015) Curr Opin Cell Biol. 36:41.

Western blot analysis of human Jurkat whole cell lysate. The blot was probed with mouse monoclonal anti-integrin αL (IM5941) at a dilution of 1:500 (lane 1), 1:1000 (lane 2), 1:2000 (lane 3) and 1:4000 (lane 4).

Western blot analysis of human MDA-MB-231 (lane 1) and A431 (lane 2) cells. The blot was probed with mouse monoclonal anti-Integrin α2 (IM5831) at 1:1000 dilution.

Mouse monoclonal antibodies are supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.The secondary reagent is supplied in the same buffer without azide. Store all at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Product References:

MS3001 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot

This kit contains:

Integrin α2 (Extracellular region) Mouse mAb50 μlWB, EHu, Rt, Ms150
Integrin α5 (Extracellular region) Mouse mAb50 μlWB, E, IPHu150
Integrin αL (Extracellular region) Mouse mAb 50 μlWB, E, ICCHu, Rt, Ms180
Integrin αV (Extracellular region) Mouse mAb50 μlWB, EHu, Rt, Ms125
Anti-Mouse Ig:HRP Donkey pAb100 μlWB, EMs

The Integrin α family antibody sampler kit can be used to detect the expression level of Integrin α2, Integrin α5, Integrin αL, and Integrin αV. The kit includes high affinity mouse monoclonal antibodies to examine Integrin α expression levels in western blot, immunoprecipitation, and immunocytochemistry.