Catalog # AP5571

AIFM1 (C-terminal region) Antibody

Rabbit Polyclonal

Application Dilution
ELISA1:1000
WB1:500
ICC1:100

Size 100 μl

Species Reactivity Hu, Rt, Ms

MW 66 kDa

DATASHEET  




$245

Apoptosis-inducing factor (AIFM1, AIF, PDCD8) is a ubiquitously expressed flavoprotein that plays a critical role in caspase-independent apoptosis. AIF is expressed as a 66 kDa precursor protein before being N-terminally cleaved to 62 kDa and localized to the mitochondrial intermembrane space. In response to apoptotic stimuli, AIFM1 is released from the mitochondrial intermembrane as a 57 kDa fragment that can translocate to the nucleus. Treatment of isolated nuclei with recombinant AIF leads to early apoptotic events, such as chromatin condensation and large-scale DNA fragmentation. Studies of AIF knockout mice have shown that the apoptotic activity of AIF is cell type and stimuli-dependent. Also noted was that AIF was required for embryoid body cavitation, representing the first wave of programmed cell death during embryonic morphogenesis. Structural analysis of AIF revealed two regions, an oxidoreductase activity and a DNA binding domain. AIF has dual functions, a pro-apoptotic activity in the nucleus via its DNA binding and an anti-apoptotic activity via the scavenging of free radicals through its oxidoreductase activity.

 

References
Delavallee, L. et al. (2011) IUBMB Life. 63(4):221.
Lipton, S.A. and Bossy-Wetzel, E. (2002) Cell 111:147.
Daugas, E. et al. (2000) FEBS Lett. 476:118.

Western blot image of human jurkat cells untreated (lanes 1 & 3) or treated with calyculin A (100 nM, 30 min.) (lanes 2 and 4). The blot was probed with rabbit polyclonals anti-AIFM1 (C-terminal region) at 1:500 (lanes 1 & 2) and anti-AIFM1 (Ser-116) phospho-specific antibody at 1:1000 (lanes 3 & 4).

Immunocytochemical labeling of AIFM1 in aldehyde fixed and NP-40 permeabilized human NCI-H446 lung carcinoma cells. The cells were labeled with rabbit polyclonal anti-AIFM1 (AP5571). The antibody was detected using goat anti-rabbit DyLight® 594.



AIFM1 (C-terminal region) synthetic peptide (coupled to carrier) corresponding to amino acids in the C-terminal half of human AIFM1. This sequence is identical in rat and mouse AIFM1, and has low homology to AIFM2 and AIFM3.

*For more information, see UniProt Accession O95831
Rabbit polyclonal, affinity-purified antibody is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

The antibody was purified by ion exchange chromatography. This antibody antibody detects a triplet at 66 and 62 kDa* protein on SDS-PAGE immunoblots of human Jurkat and K562 cells, as well as mouse C2C12 cells.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains:

KIT SUMMARY