Catalog # AM4261


α-Actinin (C-terminal region)

Mouse Monoclonal

Application Dilution
ELISA1:2000
IP1:100
WB1:1000

Size 100 μl

Species Reactivity Hu, Rt, Ms

MW 104 kDa

Isotype IgG1

$245

α-Actinins are widely expressed cytoskeletal proteins that cross-link actin filaments through anti-parallel homodimers of the rod domains. Four α-actinin genes have been discovered in humans with α-actinin 1 and 4 being widely expressed in non-muscle cells. α-Actinins contain three conserved domains that include an N-terminal actin binding domain, four spectrin-like repeats in the central region, and a C-terminal calmodulin binding domain. α-Actinin cross-links the actin filament networks and associates the network to focal adhesion sites through binding of talin and vinculin. α-Actinin 1 is phosphorylated at Tyr-12 by FAK, while α-actinin 4 can be phosphorylated at Tyr-4 and Tyr-31 after EGF treatment. Tyr-4 and Tyr-31 phosphorylation inhibit actin binding and reduces actin-filament driven multi-nucleation in rat kidney cells. Thus, phosphorylation in α-actinins may be important for regulating actin binding and actin cytoskeletal remodeling.

 

References

Shao, H. et al. (2010) J Biol Chem. 285(4):2591.
Izaguirre, G. et al. (2001) J Biol Chem. 276(31):28676.
Ylanne et al. (2001) Structure. 9:597.

Clone M426 was generated from a recombinant protein containing amino acids in the C-terminal region of human α-actinin 1. This sequence is well conserved in rat and mouse α-actinin 1, and is also conserved in α-actinin 2, 3, and 4.

*For more information, see UniProt Accession P12814
Mouse monoclonal antibody purified with protein A chromatography is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

The antibody detects a 100 kDa* protein corresponding to the molecular mass of α-actinins on SDS-PAGE immunoblots of human A431 and Jurkat cells, as well as rabbit spleen fibroblasts. The antibody may detect all four α-actinin family members.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Western blot analysis of α-actinin 4 in A431 cells stimulated with pervanadate (1 mM) for 30 min (lanes 1,2,5.6) or after immunoprecipitation using α-actinin (C-terminal region) antibody in the absence (lanes 3 & 7) or presence of pervanadate-treated A431 cell lysate (lanes 4 & 8). Some lanes of the blot were treated with alkaline phosphatase (lanes 2 & 6). The blots were probed with anti-α-actinin (C-terminal region) or anti-α-actinin 4 (Tyr-4).



This kit contains:

KIT SUMMARY