Catalog # AM3341


Abp1 (C-terminal region)

Mouse Monoclonal

Application Dilution
ELISA1:2000
ICC1:100
WB1:1000

Size 100 μl

Species Reactivity Hu

MW 55 kDa

Isotype IgG1

$245

Cellular morphology, adhesion, and motility occur through dynamic reorganization of actin-based superstructures. Actin-binding proteins are critical for regulating actin polymerization and superstructure formation. The Arp2/3 complex is an actin polymerization-inducing complex that includes Arp2, Arp3, p41-Arc, p34-Arc, p21-Arc, p20 Arc, and p16-Arc. Several actin binding proteins, WASP, cortactin, and Abp1, regulate the activity of the Arp2/3 complex. Abp1 (SH3P7, HIP-55) is the mammalian homologue of the yeast Abp1p, which has been shown to bind actin and to have roles in endocytosis. Abp1 contains a C-terminal Src homology 3 domain and an N-terminal actin depolymerization factor (ADF-H/C) domain that is homologous to a similar domain found in the actin severing protein, cofilin. Abp1 can bind Src kinases, hematopoietic progenitor kinase 1 (HPK-1), myosin I heavy chain kinase, and dynamin. Thus, Abp1 may be important for regulation of the actin cytoskeleton through its ability to interact with F-actin and various other cytoskeletal and cell signaling proteins.

 

References
Kessels, M.M. et al. (2001) J Cell Biol. 153(2):351.
Olazabal, I.M. & Machesky, L.M. (2001) J Cell Biol. 154(4):679.
Larbolette, O. et al. (1999) Mol Cell Biol. 19(2):1539.
Clone (M334) was generated from a recombinant sequence that included amino acids in the C-terminal region of human Abp1.

*For more information, see UniProt Accession Q9UJU6
Mouse monoclonal, protein A purified antibody is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at -20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

This antibody detects a 55 kDa* protein corresponding to the molecular mass of Abp1 on SDS-PAGE immunoblots of human Jurkat, Hela, and A431 cells, but does not detect Abp1 in mouse brain.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Western blot of Jurkat (lane 1), HeLa (lane 2), mouse brain (lane 3), and A431 (lane 4). The blot was probed with mouse monoclonal anti-Abp1 (C-terminal region) antibody at 1:500.

Immunocytochemical labeling of Abp1 in aldehyde-fixed and NP-40-permeabilized differentiated A431 cells. The cells were labeled with mouse monoclonal anti-Abp1 (AM3341) antibody, then the antibody was detected using appropriate secondary antibody conjugated to DyLight® 594. The cells were counterstained with Sytox green to label nuclei.



This kit contains:

KIT SUMMARY